Early Drosophila Spaghetti-Squash-GFP FRAP

The actomyosin cytoskeleton is a crucial driver of morphogenesis. Yet how the behavior of large-scale cytoskeletal patterns in deforming tissues emerges from the interplay of geometry, genetics, and mechanics remains incompletely understood. Convergent extension flow in D. melanogaster embryos provides the opportunity to establish a quantitative understanding of the dynamics of anisotropic non-muscle myosin II. Cell-scale analysis of protein localization in fixed embryos suggests that there are complex rules governing how the control of myosin anisotropy is regulated by gene expression patterns. However, technical limitations have impeded quantitative and dynamic studies of this process at the whole embryo level, leaving the role of geometry open. Here we combine in toto live imaging with quantitative analysis of molecular dynamics to characterize the distribution of myosin anisotropy and corresponding genetic patterning. We found pair rule gene expression continuously deformed, flowing..., Fluorescence recovery after photobleaching (FRAP) of junctional myosin-II in the early Drosophila embryo. Data for the manuscript \"Geometric control of Myosin-II orientation during axis elongation\" by Lefebvre & Claussen et al.
Data consists of .tif stacks showing a full-time series of a FRAP experiment carried out on a Leica SP5 confocal microscope with a 63x/1.4 NA oil immersion objective at a frame rate of 1 frame per 1.78 seconds. Regions were bleached with 50mW laser power. , Software to view .tif files, e.g. Fiji.Â