Integration of CRISPR/Cas9 expression vector into porcine genome.
收藏Figshare2016-01-18 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Generation_of_Knock_In_Pigs_Carrying_Oct4_tdTomato_Reporter_through_CRISPR_Cas9_Mediated_Genome_Engineering_/1636939
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资源简介：
(A) Genome PCR for integration of Cas9 sequence in selected clones C51, C95 and C98. Markers: DL2000; Cas9 PFFs: the porcine fetal fibroblasts transient transfected with CRISPR/Cas9 expression vector; WT PFFs: wild type PFFs. (B) Genome PCR for integration of Cas9 sequence in fetuses and piglets. Markers: DL2000; Cas9 PFFs: the porcine fetal fibroblasts transient transfected with CRISPR/Cas9 expression vector; WT PFFs: wild type PFFs. (C) Fetal and piglet fibroblasts from C95 clones cultured in medium containing G418 (800 μg/mL) for 7 days. All fetal and piglet fibroblasts from C95 clones grew well in G418 medium. Wild type PFFs, as control, died all after cultured in G418 medium for 7 days. (PDF)
创建时间：
2016-01-18
