Effect of Lactate dehydrogenase inhibitor (LDHi; GSK2837808A) on BCG vaccine induced trained immunity in vitro

Trained immunity is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to certain microbial components, altering their responses to future exposures. This study examined the in vitro effect of an LDH inhibitor on BCG induced trained immunity in human primary monocytes. Overall design: Monocytes from four donors were cultured in medium or stimulated with BCG for 24h in the presence or absence of LDH inhibitor, followed by treatment with or without LDH inhibitor for 5 more days. At day 6 macrophages were washed and stimulated with LPS or cultured in media for 4h followed by transcriptome analysis with RNA-seq.