Soft X-ray tomography reveals variations in B.subtilis biofilm structure upon tasA deletion [Data]

Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-capillary” growth method compatible with full-rotation soft X-ray tomography, providing high-resolution 3D imaging of bacterial cells and their surrounding extracellular matrix during biofilm formation, without drying or fixation steps. These datasets are reconstructed 3D volumes obtained by soft X-ray tomography of WT and ΔtasA mutants, including rescue with TasA protein. <br/>
The data is organized in individual *.tif files with prefixes as follows:<br/>
<ul>
<li>for single-cell suspension WT "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_0h_10797";
</li><li>for single-cell suspension ΔtasA mutants "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_0h_10801_";
</li><li>for WT biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_10479";</li><li>for ΔtasA biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_10517";</li><li>for ΔtasA biofilm formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_ctrl_44h_10815";</li><li>for ΔtasA biofilm with the addition of TasA protein formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_rescue_44h_10821"</li></ul>