FRET analyses of positive FRET samples of MBP-IIGlc-CFP/YFP of peak 1 after digestion with factor Xa.
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aThe presence of FRET was indicated by a decrease (>10%) in the ratio of emission at λ 476/emission at λ 526 for the corrected CFP (CFP in the CFP and YFP mixture after subtraction of the contribution due to YFP) as compared to the ratio of emission at λ 476/emission at λ 526 for CFP alone (control).
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2015-12-02



