Transcriptomic analysis of Notch (N) induced [NΔecd] Drosophila larval primary CNS tumours vs N allograft tumours in w1118 host flies

The goal of this project was to compare NGS derived transciptional profiles of primary larval CNS tumours and allograft tumours from two stages (first-T0 and forth-T3) in Drosophila that are induced by overactivation of N pathway in order to decipher if N regulates similar or different sets of genes as primary hypeplasias transition to malignant tumours. The results of this study show that overactivation of N negatively regulates genes related to neuronal differentiation processes genes as N hyperplasias become more malignant and that genes related to stress, metabolsim and immunity are upregulated mRNA profiles from FACS sorted, GFP positive Drosophila larval CNS N tumours (N FACS) vs the first (T0) allograft N tumours (N T0) vs the fourth (T3) serial allograft N tumours (N T3)