small RNA profiling of extracellular RNA from breast cancer cell lines

We performed small RNA sequencing of conditioned media collected from breast cancer cells as well as human mammary epithelial cells (HUMEC) in biological replicates. Overall design: BRCA-lines_CM_smRNA: Eight breast cancer lines and human mammary epithelial cells were cultured in biological replicates. RNA was then extracted from conditioned media (CM) and subjected to small RNA sequencing. The number of unique orphan non-coding RNAs were then recorded for each sample.