AHR activation in glioblastoma cells [FICZ_KynA]

The objective of this study was to analyze AHR activation through aromatic amino acid metabolism. To this end, glioblastoma cells were exposed to aromatic amino acid derived metabolites and their ability to activate AHR was analysed. In addition, AHR activation was evaluated in glioblastoma cells expressing IL4I1, an aromatic amino acid degrading enzyme, with or without shRNA mediated knockdown of AHR. FICZ_KynA_U87_shC: expression profile of shCtrl U-87MG cells treated with 6-formylindolo(3,2-b)carbazole (FICZ) 100 nM or kynurenic acid (KynA) 50 μM for 24 h, was compared to expression profile of shCtrl U-87MG cells treated with DMSO (n = 4).