List of nucleotide polymorphisms in HSP17.8 and their effects on codon frequencies.

aFirst letter indicates common bp at this site, followed by position of SNP in sequence on GenBank accession number AK368988.1, and then nucleotide which is the rare variant at this site.bAll nucleotide changes identified by sequencing were first by EcoTILLING as a band on gel image. In one sample, 407 bp and 394 bp were identified on EcoTILLING gel for which a corresponding polymorphism could not be confirmed by sequencing.cFrequency was calculated by dividing the number of similar nucleotide changes identified on EcoTILLING gel by the number of samples analyzed.dFirst letter indicates the common amino acid at this site, followed by position of SNP within predicted protein sequence and then amino acid change induced by the variant nucleotide polymorphism. ‘ = ’ means no change in amino acid encoded by that codon (synonymous variation).eA non-synonymous SNP is predicted to be damaging to encoded protein if PARSESNP score is >10 (bold).fA non-synonymous SNP is predicted to be damaging to encoded protein if SIFT score is gPutative polymorphisms in gel regions with high levels of noise from primer mispriming, one fragment evidently appeared on one image channel and the corresponding fragment in alternative image channels could not be unambiguously assigned.