H3K9-butyryl (H3K9Bu) and H3K9-acetyi (H3K9ac) genome patterns before and after transverse aortic constriction in Balb/cJ and Balc/cByJ mice

Balb/cJ mice on a low-fat diet show a decline in promoter H3K9-butyryl (H3K9Bu) in the heart, after subjecting them to transverse aortic constriction (TAC). In contrast, Balb/cByJ, which has a deletion in the ACADS gene, exhibit an increase in promoter H3K9Bu. Conversely, H3K9ac increases in the former and declines in the latter. Overall design: Balb/cJ and Balb/cByJ mice were fed either a low-fat (10 Kcal%) diet for 4 days before subjecting them to a sham or transverse aortic constriction (TAC) surgeries. The mice were maintained on the same diet for another week, before they were assessed by echocardiography, the hearts isolated, chromatin extracted , and subjected to anti-H3K9Bu and anti-H3K9ac ChIP-Seq