Bos taurus breed:Holstein Transcriptome or Gene expression

Johne’s disease is a chronic infection of the intestinal mucosa caused by Mycobacterium avium subspecies paratuberculosis (MAP). The events of pathogen survival, inflammation and progression to the clinical stages of infection are poorly understood. This study examines gene expression in the ileoceocal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and provides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared from ICV tissues and RNA-Seq was used to compare gene transcription between clinical, subclinical, and negative control animals.

约翰氏病是由副结核分枝杆菌（Mycobacterium avium subspecies paratuberculosis，简称MAP）引起的慢性肠道黏膜感染。病原体存活、炎症发生以及感染进展至临床阶段的病理过程尚不明确。本研究旨在探究荷斯坦奶牛在不同MAP感染阶段的回盲瓣（ICV）基因表达情况。回盲瓣被认为是MAP定植的主要部位，并提供了理想的位置以识别与该疾病进展相关的基因。从ICV组织中制备RNA，并利用RNA-Seq技术对比临床、亚临床和阴性对照组动物之间的基因转录差异。