RNA sequencing analysis of 5637 cells with D-xyl@epsilonPLCDs

We designed D-xylose-modified, epsilon-poly-L-lysine-based carbon dots (D-xyl@epsilonPLCDs) to effectively inhibit intracellular UPEC. D-xyl@εPLCDs effectively entered cells, reduced bacterial adhesion and promoted intracellular UPEC clearance. To investigate the regulation of immune responses in bladder epithelial 5637 cells after treatment with D-xyl@epsilonPLCDs, RNA sequencing analysis were performed. Human bladder epithelial 5637 cells were treated with either epsilonPLCDs, D-xyl@epsilonPLCDs or sterilized water for 3 hours. The cells were harvested and subjected to RNA extraction using TRIzol methods followed by deep RNA sequencing. Subsequently, we performed gene expression profile analysis based on RNA sequencing data obtained from 5637 cells in three groups (Control, epsilonPLCDs and D-xyl@epsilonPLCDs).