Experimental dataset related to the study of the impact of whole-life exposure to ocean acidification on puberty and reproduction of the European sea bass

the dataset reported here corresponds to the experimental study aiming at evaluating the impact of whole-life acclimation to different degrees of ocean ph/pco2 (current condition of the rade de brest and predictions of rcp8.5 ipcc scenario by 2050 and by 2100) on the sexual maturation and spawning of a temperate marine teleost, the european sea bass (dicentrarchus labrax), over two consecutive reproductive cycles.the hypothesis that severe acidification would lead to the highest impact on plasma sex steroids concentrations and on spawning phenology driving to higher female fecundity was tested. the same physiological traits were monitored during the two first reproductive seasons to estimate the acclimation potential at longer exposition, in pubertal and post-pubertal fish.in all data sheets, “id” indicates the identification number of fish and “scenario” the corresponding acidification level. the dataset contains:- fulton’s condition factor (k) of females and males in both reproductive seasons individually determined from gametogenesis (november) to spawning season (february to april).- female 17β-estradiol (e2) and male 11-keto testosterone (kt) and testosterone (t) plasma concentrations (expressed as ng/ml), during the two reproductive seasons (from gametogenesis (november) to post-spawning season (april)). sexual steroids concentrations were measured by elisa with kit from cayman chemical (usa, kits # 582701, # 582751, #501890 for testosterone, 11-keto testosterone and 17β-estradiol quantification respectively).- proportion of the oocytes at each stages of development (freq) at the beginning of the spawning period (in february and march) of the second reproductive cycle. the classification of stages of oocytes development (stage) followed the criteria previously reported for european sea bass (mylonas et al. 2003) with few modifications: previtellogenic (no ongoing vitellogenesis with diameter > 700 µm); stage a (vitellogenic oocytes with central germinal vesicle and no lipid-droplet coalescence); stage b (post-vitellogenic oocytes showing hyalinisation of the periphery of the cytoplasm); stage c (central germinal vesicle and early lipid-droplet coalescence); stage d (migration of the germinal vesicle and lipid-droplet coalescence) and hydrated eggs (fully hydrated eggs ready to be spawned). for statistical analysis, the earliest developmental stages (previtellogenic, a and b) and the latest ones (stages c, d and hydrated) were pooled in two groups named ‘stage 1’ and ‘stage 2’ respectively. counts for each stages (stage1 and stage2) as well as the frequency of the stage 2 (freq_2) per female is reported.- proportions of fluent males (sperm) per condition from february to april of the first (1) and second (2) reproductive season.- number of eggs spawn per female (egg_female) and per day from february to april of the two reproductive seasons.”day_of_spawning” indicates per each date if it was a spawning day (y) or not (n) per each scenario;- total number of eggs spawn per female (total egg#/female) during the two reproductive seasons (relative fecundity).- physico-chemical parameters of the rearing water during the two reproductive seasons including the carbonate system components. for previous data concerning the physico-chemical water parameters of rearing tanks refer to (cohen-rengifo et al. 2022). water ph (ph nist) and temperature (t) were daily monitored by wtw 3110 ph meters (xylem analytics germany, weilheim, germany; with electrode: wtw sentix 41, nbs scale). the ph meters were daily calibrated with nbs certified wtw technical buffers at ph 4.01 and ph 7.00 (xylem analytics germany, weilheim, germany). the total alkalinity (ta) of each tank was measured once a week following the protocol described previously (cominassi et al. 2019) adapted from the protocol of anderson and robinson (1946) and strickland and parsons (1972). seawater ph in total scale and pco2 were calculated using the microsoft excel macro co2sys (lewis 1998) with the constants after mehrbach et al. (mehrbach et al. 1973), refit by dickson et al. (dickson and millero 1987) (as cited in co2sys). pco2 and fco2 values (µatm) were calculated from measured ph values in nsb scale, total alkalinity (µmol/kgsw), temperature (°c), oxygen content (%) and salinity (‰). afterwards, total scale ph values were calculated with the same macro using pco2 and fco2 values.referencesanderson, d. h. & r. j. robinson (1946) rapid electrometric determination of alkalinity of sea water using glass electrode. industrial & engineering chemistry analytical edition, 18, 767-769.cohen-rengifo, m., m. danion, a.-a. gonzalez, m.-l. begout, a. cormier, c. noel, j. cabon, t. vitre, f. c. mark & d. mazurais. 2022. partial raw data of the carbonate system after years of transgenerational exposure to ocean acidification in the european sea bass dicentrarchus labrax.[...]

本数据集所记录的实验研究旨在评估鱼类对海洋pH/PCO2不同程度的全生命周期适应（包括拉德德布斯特海域的当前状况以及至2050年和2100年根据IPCC RCP8.5情景预测的情况）对温带海洋硬骨鱼类——欧洲鲈鱼（Dicentrarchus labrax）的性成熟和繁殖产生的影响。研究假设严重酸化将导致血浆性激素浓度最高，并推动繁殖节律，进而提高雌性产卵量。在两个连续的繁殖季节中，对相同的生理特征进行了监测，以估算在较长时间暴露下的适应潜力，特别是在青春期末和青春期末的鱼类中。在所有数据表中，“id”代表鱼类的识别号码，“scenario”代表相应的酸化水平。该数据集包含以下内容：- 从精巢发生（11月）到产卵期（2月至4月）两个繁殖季节中雌性和雄性个体的富尔顿状况系数（k）；- 女性雌二醇（17β-E2）和男性11-酮睾酮（KT）及睾酮（T）的血浆浓度（以ng/ml表示），在两个繁殖季节（从精巢发生（11月）到产后季节（4月））；性激素浓度通过酶联免疫吸附测定（ELISA）使用Cayman化学（美国，套件编号# 582701、# 582751、#501890用于睾酮、11-酮睾酮和17β-雌二醇定量）进行测量。- 第二个繁殖周期产卵期开始时（2月和3月）每个发育阶段卵母细胞的比例（freq）；卵母细胞发育阶段的分类（阶段）遵循先前报道的欧洲鲈鱼标准（Mylonas等，2003年），略有修改：前卵黄生成期（无进行中的卵黄生成，直径> 700 µm）；阶段A（卵黄生成卵母细胞具有中央生发泡且无脂滴融合）；阶段B（后卵黄生成卵母细胞显示细胞质外围的透明化）；阶段C（中央生发泡和早期脂滴融合）；阶段D（生发泡迁移和脂滴融合）以及孵化卵（完全水化的准备产卵的卵）；为了统计分析，最早发育阶段（前卵黄生成期、A和B）和最晚阶段（C、D和孵化）分别合并为两组，分别命名为“阶段1”和“阶段2”。报告了每个阶段（阶段1和阶段2）的计数以及每个雌性的阶段2频率（freq_2）。- 从2月到4月，每个条件下流畅雄性（精子）的比例在第一个（1）和第二个（2）繁殖季节。- 每个雌性产卵数量（egg_female）以及两个繁殖季节（2月到4月）每天产卵数量；产卵日（day_of_spawning）表示在每个日期，针对每个情景是否为产卵日（y）或非产卵日（n）；- 两个繁殖季节中每个雌性的总产卵数量（total egg#/female）（相对产卵量）；- 两个繁殖季节中养殖水体的物理化学参数，包括碳酸盐系统成分。关于养殖水箱的物理化学水参数的先前数据，请参阅（Cohen-Reنگifo等，2022年）。pH（pH NIST）和温度（t）通过WTW 3110 pH计（Xylem Analytics德国，Weilheim，德国；电极：WTW Sentix 41，NBS标度）每天监测。pH计每天使用NBS认证的WTW技术缓冲液在pH 4.01和pH 7.00（Xylem Analytics德国，Weilheim，德国）进行校准。每个水箱的总碱度（TA）每周测量一次，遵循之前描述的方案（Cominassi等，2019年），该方案改编自Anderson和Robinson（1946年）以及Strickland和Parsons（1972年）的方案。海水pH的总标度和PCO2使用Microsoft Excel宏CO2sys（Lewis，1998年）计算，使用 Mehrbach等（Mehrbach等，1973年）的常数，经Dickson等（Dickson和Millero，1987年）校准（如CO2sys所述）。PCO2和fCO2值（µatm）通过在NBS标度中测量的pH值、总碱度（µmol/kgsw）、温度（°C）、氧气含量（%）和盐度（‰）计算得出。之后，使用相同的宏计算总标度pH值。参考文献：Anderson, D. H. & R. J. Robinson (1946) 快速电化学测定海水碱度使用玻璃电极。工业与工程化学分析版，18，767-769。Cohen-Reงifo, M.，M. Danion，A.-A. Gonzalez，M.-L. Begout，A. Cormier，C. Noel，J. Cabon，T. Vitre，F. C. Mark & D. Mazurais. 2022. 欧洲鲈鱼Dicentrarchus labrax代际暴露于海洋酸化多年后碳酸盐系统的部分原始数据。[...]