Genome-wide maps of histone H3 variant CENP-A/Cnp1 in the meiotic haploid progeny of wip1∆/+, mhf1∆/+ and mhf2∆/+ and genome-wide maps of histone H3 variant CENP-A/Cnp1 in mhf2+ (or mhf2∆) carrying the inactivated Centromere 1 or Centromere 2.

We report the high-throughput profiling of histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide CENP-A/Cnp1 maps of the wild type meiotic haploid progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+ and CENP-A/Cnp1 maps of mhf2+ carrying the inactivated Centromere 1 or Centromere 2. We find that CENP-A/Cnp1 spreading into the pericentromeric regions in wild type meiotic progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+. 4 wild type meiotic haploid progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+ were examined by anti-CENP-A/Cnp1 ChIP-seq. And biological replicates of mhf2+ carrying the inactivated Centromere 1 or Centromere 2 were examined by anti-CENP-A/Cnp1 ChIP-seq.