Transcriptome analysis reveals miRNA expression profiles in hypothalamus tissues during the sexual development of Jining grey goats

In order to reveal the changes of microRNA spectrum in hypothalamus tissues of goats from birth to sexual maturity, smallRNA sequencing was performed on hypothalamus tissues of Jining grey goats at 4 developmental stages after birth. Twenty libraries from 4 different developmental stages (5 goats per stage) were successfully constructed and the corresponding miRNA expression profiles were obtained. Overall design: To investigate the dynamics of hypothalamic microRNA expression profiles at four developmental stages after birth, we performed smallRNA sequencing using hypothalamic tissue from goats at four developmental stages with five biological replicates per developmental stage. Under the same breeding environment and management conditions, all goats have free access to food and water.