Establishing Bovine Embryonic Stem Cells and Analyzing Potential Mechanisms of their Self-Renewal

Bovine pluripotent stem cells (PSCs) hold significant potential for diverse applications in agriculture, reproductive biotechnology, and biomedical research. However, challenges persist in establishing stable bovine PSC lines and understanding the mechanisms un-derlying their pluripotency maintenance. Here, we derived bovine embryonic stem cells (bESCs) from Holstein cattle embryos. These cells exhibited robust differentiation capacity into three germ layers in vitro and in vivo. Transcriptome analysis revealed distinct molecular profiles compared to primed-state bESCs. Notably, bESC proliferation ceased on methanol-treated feeder cells, in contrast to mouse ESCs (mESCs), which proliferated normally. Pathway analysis identified key signaling events critical for bESC survival and proliferation, highlighting species-specific regulatory mechanisms. Furthermore, the derived bESCs demonstrated chimerism capacity in early bovine embryos, underscoring their functional pluripotency. This work provides a foundation for advancing bovine embryology research and stem cell-based biotechnologies in livestock. Overall design: RNAseq profiling of our established bESCs and their comparison with the reported RNAseq data from bMSCs (GSM5478616,GSM5478617,GSM5478618), biPSCs(GSM5478624,GSM5478625), primed state bESCs (GSM2976768,GSM2976773), and bovine blastocysts (GSM1265773,GSM1265774,GSM1265775). RNAseq profiling and comparing of bovine ESCs or mouse ESCs cultured on methanol-treated mouse embryonic fibroblasts (MEFs) or regular MEF feeders. *************************************************************** The table below lists GEO accessions reused/reanalyzed for this study. ***************************************************************