Supplemental Material for Generation of Oviductal Glycoprotein 1 Cre Mouse Model for the Study of Secretory Epithelial Cells of the Oviduct

Supplemental Materials: Generation of Oviductal Glycoprotein 1 Cre Mouse Model for the Study of Secretory Epithelial Cells of the Oviduct

Supplemental Figure S1: Expression validation of PGR in Pgrf/f and Ovgp1Cre:eGFP/+;Pgrf/f oviducts using PGR immunohistochemical analysis. The oviducts were collected from Pgrf/f and Ovgp1Cre:eGFP/+;Pgrf/f female mice at 8 weeks old. Oviduct tissues were immunostained with anti-PGR (D8Q2J) XP rabbit monoclonal antibody (Cell Signaling Technology, #8757) with a dilution of 1:1200. Expression of PGR in Pgrf/f and Ovgp1Cre:eGFP/+;Pgrf/f oviducts at (A) infundibulum, ampulla, and isthmus regions and (B) uterotubal junction (UTJ) adjacent to the uterus. Insets are higher magnifications of the UTJ and uterus. (C) Negative control images from the oviduct and UTJ+uterus without the primary antibody. All samples were collected at the estrus stage. Scale bars = 50 m, n = 3 mice/genotype. The staining protocol was identical to the original antibody listed in the method, except for the primary antibody and its dilution factor.