Single cell RNA-sequencing (scRNA-seq) of ISL1 knockout (KO) or knockdown and NKX2.5 KO compared to WT in cardiac progenitors (CPs); scRNA-seq of ISL1 KO compared to WT in motor neuron progenitors (MNPs)

Previous evidence suggested ISL1 plays unique transcriptional roles between vastly different cell types in developing humans. Single cell RNA-sequencing (scRNA-seq) of in vitro progenitor cells detailed ISL1's cell-type-specific roles in human cardiac progenitors (CPs) and motor neuron progenitors (MNPs). In addition, scRNA-seq confirmed ISL1 and NKX2.5 likely regulate common transcriptional pathways. Overall design: scRNA-seq of WT, ISL1 KO, NKX2.5 KO in D8 CPs (n=3). scRNA-seq of WT vs ISL1 KO in D18 MNPs (n=3). scRNA-seq of scrambled control vs siRNA ISL1 in D8 CPs (n=1).