Strains used in this study are listed with unique identifiers (SGM-#). For E. coli donor strains, the primers or gene block used to construct each plasmid are listed in the “Oligos” column. Genetic ch
(a) 5’tetA and the 3’tetA contain the 1–779 and the 342–1190 part of the tetA coding region, respectively. They contain a 342–779 overlapping region. The positions are numbered relatively to the first
Three primers were used to sequence the insert in the ∆ rv1878 complementation construct.Last file is the in silico designed construct (with added optimized ribosomal binding site) used for scre
