Gene expression induced by DOT1L and Menin inhibition in cell line models of leukemia. Gene expression induced by DOT1L and Menin inhibition in cell line models of leukemia

Gene expression upon DOT1L inhibition, or Menin inhibition, or a combination of DOT1L and Menin inhibiting agents, was assessed in several MLL-rearranged human cell lines and a mouse model of MLL-AF9 leukemia. The goal of the study was to explore the mechanisms by which the EPZ0004777 and MI-2-2 chemicals collaborate to induce differentiation and cell death in MLL-AF4 and MLL-AF9 leukemias. Overall design: 2 compounds and combinaison thereof, 1 concentration & 1 timepoint per treatment, DMSO control, 3 biological replicates