Determination of the RNA linked to the paraspeckle in the GH4C1 cell line. Rattus norvegicus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA323540
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资源简介：
The goal of this study was to determine the identity of the RNA linked to the paraspeckles in the rat GH4C1 cell line. Overall design: GH4C1 cell were fixed with 1% paraformaldehyde in PBS. Then nuclei were purified, lysed and sonicated. RNA pull-down was performed using two antisense DNA biotinylated oligonucleotide probes that target the lncRNA Neat1. Streptavidin-magnetic beads were added to hybridization reaction and complexes were captured by magnets. RNA was isolated using NucleoSpin®RNA XS (Macherey-Nagel).
创建时间：
2016-05-27
