Expression Analysis of 2D, 3D Quiescent, and 3D Activated Pancreatic Stellate Cells. Mus musculus

This study used Illumina single-end RNA-sequencing to examine gene expression differences between 2 mouse-derived pancreatic stellate cell lines (PSC4, PSC5) grown either in 2D monolayers, as 3D quiescent cultures, or as 3D activated transwell cocultures with 2 mouse tumor-derived pancreatic ductal organoid lines (T4, T5). Mouse pancreatic stellate cell (PSC) lines were derived from the pancreata of wild-type C57Bl/6J mice. Mouse tumor organoid lines were derived from mouse pancreata containing pancreatic ductal adenocarcinoma (PDAC) from the KrasLSL-G12D; Trp53LSL-R162H; Pdx1-Cre mouse model. We measured genes differentially expressed among 2D, quiescent, and 3D activated PSCs that may reflect the expression changes of heterogeneous CAF population in pancreatic tumors. Overall design: Gene expression comparison of 2 murine pancreatic stellate cell lines grown either in 2D monolayers, 3D quiescent cultures, or 3D activated transwell cocultures with 2 murine pancreatic ductal adenocarcinoma-derived pancreatic ductal organoid lines.