Mitochondrial Dynamics Govern Whole-body Regeneration Through Stem Cell Pluripotency and Mitonuclear Balance [RNAi scRNA-seq]

To further identity the relationship between neoblast pluripotency and mitochondrial dynamics in cell lineage commitment,single-cell RNA sequencing was used to further analyze the compositions of mito low cells. To further identify the relationship between all different tissues cells and mito chondrial dynamics, snRNA-seq was used to further analyisis. Isolation by flow cytometry to get the mito low cells in RNAi planarians. Isolation by flow cytometry to get the mito low cells in RNAi planarians.Isolating the cell nuclei in RNAi planarians.