Identification of genes enriched in Nkx2-1-positive neurons in the Arc, VMH, and DMH of adult mice

We aimed to identify genes enriched in Nkx2-1-positive neurons in the dorsomedial hypothalamus (DMH). Using Nkx2-1-CreERT2 mice crossed with Rosa-ZsGreen mice after tamoxifen induction, we collected ZsGreen-marked Nkx2-1-positive neurons from the DMH, ventromedial hypothalamic nucleus (VMH), and arcuate nucleus (Arc) by laser microdissection and compared their gene expression profiles. Nkx2-1-positive neurons (marked by ZsGreen) in the Arc, VMH and DMH were collected from 2-month-old Nkx2-1-CreERT2 mice crossed with ROSA-loxSTOPlox-ZsGreen1 mice after tamoxifen injection by laser micro-dissection using the Leica LMD 6000 system (n=2-3 each group).