DNA sequencing of the 1st generation of Rocky Mountain spotted fever vaccine made by Rocky Mountain Laboratories in 1944

An unopened Rocky Mountain spotted fever (RMSF) vaccine vial from Rocky Mountain Rocky Mountain Laboratories was obtained, which contains the 1st generation RMSF vaccine prepared from infected tick tissue in 1944. Illumina Next Generation Sequencing (NGS) technology was applied to the recovered DNA from this material and the obtained DNA sequence information revealed the contents of this RMSF vaccine produced in last century.