Coevolution between PPI partners detected using correlated changes in selective constraint.

aValues of branch-wise selective constraint (ω) allowed in the computation of Spearman’s correlation between these ω values between paired branches for two proteins with a known PPI in humans (Methods).bValues of ω from the indicated clades were removed before the calculation of the Spearman’s correlation.cWe required at least 6 common branches between the two orthologous genes trees for the two interacting proteins: the column indicates the number of PPIs meeting this requirement.dP-value of the hypothesis test that the real PPI pairs had a higher mean Spearman’s correlation than would be expected, given the distribution of correlations seen from 1000 simulations of the same number of pseudo-PPI pairs drawn from non-interacting proteins (Methods).eMean of the mean correlation seen from 1000 simulations, each consisting of the same number of pseudo-PPIs from c.