Molecular architecture of transcription factor hotspots in early adipogenesis. Mus musculus

Here, we have used digital genomic footprinting to precisely define protein localization for several adipogenic transcription factors at a genome-wide level. In combination with ChIP-seq data, these analyses reveal novel molecular insight into the organization of transcription factors at hotpot regions, which provides a new framework for understanding transcription factor cooperativity on chromatin. Overall design: Digital genomic footprinting and gene expression in 3T3-L1 pre-adipocytes by high throughput sequencing.