Coupled myovascular expansion directs cardiac growth and regeneration

Purpose: The goal of this experiment is to better understand the molecular mediators of myovascular coupling during growth and injury. Methods: We performed single cell RNA-sequencing (scRNA-Seq) of border zones of cryoinjured murine hearts collected at P7 using the Drop-seq protocol. Results: Together with in situ hybridization data, results from the scRNA-seq data analysis support a dynamic role for myocardial VEGFA to endothelial VEGFR2 signaling as a regulator of the myovascular expansion during growth and regeneration. Overall design: Single-cell RNA-seq through Drop-seq technique of border zones from mice hearts collected at P7 pooled into one sample