Comparison of the specific binding sites for [3H]15d-PGJ2 in plasma membranes to authentic receptors, DP1 and DP2.

Apoptotic effects of 15d-PGJ2–related compounds were correlated to their affinities for DP1, DP2 and the specific binding sites for [3H]15d-PGJ2 (SBJ). LD50: The concentration of 15d-PGJ2–related compounds required to induce apoptosis in the half of neurons which were cultured for 24h in the absence of serum. LT50: The time of 10 µM 15d-PGJ2–related compounds required to induce apoptosis in the half of neurons which were cultured in the absence of serum. IC50: The concentration of 15d-PGJ2-related compounds required to inhibit half of the specific binding of [3H]15d-PGJ2 to SBJ. LD50, LT50 and IC50 were calculated from Yagami et al.[4]. These data on Ki: The Ki values of 15d-PGJ2-related compounds to DP1 and DP2 were referred from Sawyer et al[53].