RNA isolated from infected and uninfected PBMCs was evaluated first for the level of endogenous controls by RT-PCR using specific primers and probes as a measure of RNA quality and quantity before mic
RIN values were measured using the Eukaryote Total RNA Pico assay on the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA yield is based on a sample eluate of 35 µ L. RNA concentration was measure
