Heat-shock molecular chaperone Hsp90 is involved in heterochromatin assembly in Schizosaccharomyces pombe.

Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25? and 37?. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects. Overall design: Examination of H3K9me2 in wt, ago1D and tas3D growing at 25?. Particularly, swo1-26 mutant cells are cultured under permissive condition (25?) and non-permissive condition (25? shifted to 37? for 4 h).