ChIp-Seq on Normal Human Cholangiocytes
收藏NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP451570
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资源简介：
Epithelial cells subjected to low levels of irradiation can induce DNA damage response and senescence through variety of mechanisms. We perfomed ChIP-seq for H3K27ac to investigate the gene pathways activated to induce senescence in cholangiocytes. Overall design: We subjected normal human cholangiocytes. NHC, to 10GY irradiation, incubated them for 5 days at 37C . Cells were then crosslinked with formaldehyde for 10 mins at room temperature. The cells were then utilized for ChIP with H3K27ac antibody and subjected to sequencing
创建时间：
2023-11-09
