Comparative toxicity of Aflatoxin B1, Patulin and their biosynthetic precursors: A human intestinal microarray analysis

Patulin (PAT) and Aflatoxin B1 (AFB1) are frequent mycotoxins contaminating food and feed which demonstrate multiple toxic effects. These mycotoxins represent final product in cascades of enzymatic reactions. In order to limit the risk generated by food contamination, approaches aiming to block the biosynthesis pathway of these mycotoxins can be considered, provided that their precursors are devoid of toxicity. The aim of this study was to compare the toxicity of two precursor compounds (Versicolorin A (VERA), sterigmatocystin (STG)) to AFB1 and the precursor compound (ascladiol) to PAT. Human intestinal Caco-2 cells were exposed to 1 µM AFB1, VERA, STG or 3 µM PAT or ascladiol for 24 hours and transcription analysis was performed using an “Agilent Sureprint 63 Human GE 8x60k microarray. Human intestinal Caco-2 cells were exposed in vitro to different molecules at 1µM for AFB1, VERA, STG and 3 µM for PAT and ascladiol during 24 h. Then RNAs were extracted.