Copy-number profiling by SNP array of 4 acute myeloid leukemia (AML) cell lines. Copy-number profiling by SNP array of 4 acute myeloid leukemia (AML) cell lines

DNMT inhibitors (DNMTi) are finally approved for AML/MDS, also based on their activity in patients with high-risk cytogenetics (often monosomal karyotype) such as -5/del(5q) or -7/del(7q), often - but not always - harboring TP53-mutations. Several studies provided evidence for aberrant hypermethylation/silencing on monoallelic gene loci, including tumor suppressor genes. We hypothesized that transcriptional repression on monosomal gene loci may be preferentially reversed by DNMTi. Using an unbiased RNA-seq approach we aimed to identify preferentially regulated genes in cells monoallelic for chromosome 7q. Overall design: Affymetrix SNP arrays were performed according to the manufacturer's protocol on DNA extracted from the cultured AML cell lines UCSD-AML1, ELF-153, KG-1 and U937.