Expression data of mRNA from hearts of mice injected with ETX or PBS

ETX treatment promoted cardiomyocyte proliferation. To investigate the molecular mechanism, we performed microarray analysis to explore the molecular mechanism underlying the effect of ETX on cardiomyocyte proliferation. In this study, we sought to determine if and how metabolic reprogramming regulates cardiomyocyte proliferation. In neonatal mice, blockade of glycolysis by inhibiting glucokinase reduced cardiomyocyte proliferation, while blockade of fatty acid oxidation by inhibiting carnitine palmitoyltransferase 1 (CPT1) delayed the cell cycle arrest in cardiomyocytes. ETX (Etomoxir) is an effective inhibitor of CPT1 which is the key enzyme of fatty acid oxidation,ETX induced cardiomyocyte proliferation and improved cardiac function post-MI in adult mice. The total RNA was extracted from P14 heart tissue after treatment with vehicle (PBS) or ETX for 1 week.Then, hybridizations for cartridge arrays were set up by using GeneChip® Hybridization, Wash and Stain Kit (Affymetrix). Finally, the probe arrays were washed, stained and scanned to generate the data.