Whole-brain spontaneous GCaMP activity with NeuroPAL cell ID information of semi-restricted worms

Spontaneous neuronal activities were extracted from GCaMP and tagRFP images, covering nearly all neurons in the nematode C. elegans brain. Cell segmentation and tracking were performed using 3DeeCellTracker (https://github.com/WenChentao/3DeeCellTracker). Neuronal identity information was derived from NeuroPAL signals with the NeuroPAL Auto-ID software (https://www.yeminilab.com/neuropal) and manual corrections. During the data acquisition, worms were semi-restricted in a microfluidic device (https://www.nature.com/articles/nature06292). Note: There are occasional transient failures in neuronal tracking. We recommend researchers to use a moving median filter (e.g., ±3) on the time series data to address this issue.