AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein. AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein

We compared polyIC stimulated cells in the presence of either GFP or NS5 protein Overall design: A549 cells presence with GFP- or NS5-expressing plasmid using Polyjet (Signagen) according to manufacturer instructions, and 30 hours later stimulated with polyIC (Tocris) as previously described (Marazzi et al., 2012). At 12 hours post-stimulation, total cellular RNA was purified by RNeasy column (Qiagen).