Gene expression profile of WT and mdig/mina53 KO MDA-MB 231 breast cancer cells [RNA-Seq]

The mineral dust-induced gene (mdig, also called as MINA or RIOX2) is upregulated in breast cancer and predicts poor overall survival of breast cancer patients. Our studies suggested the anti-metastasis feature of mdig in regulating the cell motility of breast cancer cells. Here we studied the transcriptomic profile of human triple negative breast cancer cells MDA-MB-231 that were knocked out for mdig via CRISPR-Cas 9 gene editing. Our analysis identified distinct transcriptomic profiles in mdig knockout cells, where genes associated with epithelial-mesenchymal transition (EMT) and TGF-ß pathways were upregulated Overall design: Wildtype type (WT) and mdig knockout (KO) MDA-MB-231 cells were subjected to RNA-Sequencing