Nitrogen cycling functional gene abundance and potential activity in novel perennial forage cropping systems in Rwanda

This data is associated with a study entitled "Nitrogen cycling functional gene abundance and potential activity in novel perennial forage cropping systems in Rwanda", in which we sampled bulk soil from two locations in Rwanda cross a dry to rainy season transition. We evaluated the degree to which legume intercropping affected soil nitrogen status and putative biological nitrification inhibition in the companion intercrop species.

There are two data sets associated with this study:

1. The file entitle "soil_data.csv" contains all measurements related to bulk soil samples (n=335). We measured nitrification potential (NP), denitrification enzyme activity (log_dea) that was log-transformed for analysis, permanganate-oxidizable carbon (mg_kg_POXC), nitrate (mg_kg_NO3N), ammonium (mg_kg_NH4N), potentially mineralizable nitrogen (net_NH4N), pH, gravimetric water content (gwc), total extractable nitrogen (TN), and non-purgeable organic carbon (NPOC). In addition, this file contains metadata such as sampling location, plant treatment, block, and collection timepoint.

2. The file entitle "NiCE_data.csv " contains data from a high-throughput qPCR experiment in which we quantified twenty nitrogen cycle functional soil from the bulk soil samples from the previous dataset. This file contains: a qPCR assay number, the assay target gene, forward and reverse primers, soil ID number, gene concentration, Ct value, and the log10-transformed gene copy number.

本数据集与一项题为《卢旺达新型多年生饲料作物系统中氮循环功能基因丰度和潜在活性的研究》的论文相关联，本研究于卢旺达两个地点在干旱与雨季过渡期间采集了土壤样品。我们评估了豆科间作对土壤氮素状况及伴随间作物种中潜在生物硝化抑制的影响。与此研究相关的数据集有两个：

1. 标题为"soil_data.csv"的文件包含了与土壤样品（n=335）相关的所有测量数据。我们测量了硝化潜力（NP）、反硝化酶活性（log_dea，分析时进行了对数转换）、高锰酸盐可氧化碳（mg_kg_POXC）、硝酸盐（mg_kg_NO3N）、铵（mg_kg_NH4N）、潜在可矿化氮（net_NH4N）、pH值、重量水含量（gwc）、总可提取氮（TN）和非挥发性有机碳（NPOC）。此外，该文件还包含元数据，如采样地点、植物处理、地块和采集时间点。

2. 标题为"NiCE_data.csv"的文件包含了一项高通量qPCR实验的数据，该实验从前述数据集中的土壤样品中量化了二十种氮循环功能基因。该文件包含：qPCR实验编号、实验靶基因、正向和反向引物、土壤ID号码、基因浓度、Ct值以及基因拷贝数的10对数转换值。