Comparison of gene expression between high- and low-Sost/sclerostin expressing osteocytes

Single-cell sorted cells from the osteocytic cell line Ocy454 were screened for high- and low-Sost/sclerostin expression to see changes in other gene expressions related to Sost/sclerostin. Ocy454 cells were single cloned and 37 clones were tested for Sost/sclerostin expression after 7 days in culture. 2 clones (12H and 15H) with the highest Sost expression and 3 clones (9L, 4L and 24L) with low Sost expression were selected for microarray analysis.