Data for heme fractionation of <i>plasmepsin</i> KO parasites throughout the life cycle and exposed to PPQ (Fig 3D–F and Fig 4).

Tightly synchronized parasites were harvested at different time-points throughout the life cycle; the average and SD of percentage of hemozoin Fe, hemoglobin, and free heme Fe are shown for three independent experiments for parasites with a single locus (KH001_053G10), duplicated locus (KH001_053G8), G8PMII_KO, G8PMIII_KO, or G8PMII/III_KO. Statistical comparisons at each time point were performed between the single copy KH001_053G10 parasites and all other lines using two-tailed unpaired Student’s t-tests *p < 0.05; **p < 0.01. Additionally, parasites were incubated with 2 μM PPQ from 24 to 36 h post synchronization and harvested at 36 h. Statistical comparisons of PPQ-treated to untreated parasites at the 36 h timepoint from three independent biological replicates were performed using two-tailed unpaired Student’s t-tests *p < 0.05; **p < 0.01; ***p < 0.001. (XLSX)