Differential expression analysis of human brain samples with Alzheimer disease versus healthy control samples

Differential expression analysis of human brain samples with Alzheimer disease versus healthy control samples using an Agilent custom expression microarray. Brain tissue of 19 AD patients and 21 healthy controls dying without any history of neurological or psychiatric illness was used. The diagnosis of AD was made on the basis of both clinical and neuropathological evidence according to the criteria of the International Working Group (IWG) for New Research Criteria for the diagnosis of AD in the revision of 2014 (IWG-2), the NIA-AA diagnostic criteria in the revision of 201117 and the NIA-AA guidelines for the neuropathological assessment of AD. Only cases with typical AD according to IWG-2 criteria were included. All cases underwent neuropsychological assessment within the last 6 months prior to their death. Note, 4 arrays (2 AD, 2 control) were excluded from further processing due to quality issues of arrays.