Aditional file 2.

Gametocyte density of P. vivax cultured with different sources of reticulocytes in 7 days. Reticulocytes purified from peripheral blood (red line), cord blood (blue line) and hematopoietic stem cell (green line) were used to cultured fresh isolates of P. vivax (11 isolates). Reticulocytes were added to the cultures daily at a final 4% and the gametocyte density was determined for 7 days from Giemsa-stained thick smears (1 Âľl packed cells). Each line represents average parasite density from 7 days obtained from 11 P. vivax isolates. Cord blood-reticulocytes has highly expression of fetal hemoglobin but still shown better support gametocyte production in some parasite isolates (VKTS33, VKBT59, VKBT63, and VKBT81).