Depositing centromere repeats induces heritable intragenic heterochromatin establishment and spreading in Arabidopsis

This study investigated the mechanisms and novel engineering strategy of locus- specific transgenerational epigenetic inheritance by depositing centromere repeats Overall design: (Bisulfite-Seq) A. thaliana plants were growth on 1/2 X MS agar (0.8%) + 1% sucrose. 1-week-old seedlings were frozen in liquid nitrogen before DNA extraction for sequencing. (Small RNA-Seq) A. thaliana plants were grown on 1/2 X MS agar (0.8%) + 1% sucrose. 1-week-old seedlings were frozen in liquid nitrogen before RNA extraction for sequencing.