Wound Healing Assays In Nsd3Short Overexpressing H1299 Cells

<strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong> <strong>Exp022 Objective: </strong>During epithelial to mesenchymal transitioning (EMT), transcription networks become activated and cytoskeletal rearrangements take place. These events promote migratory capacity and invasiveness. A common assay to measure this phenotype is wound healing. This involves plating cells and culturing until confluent at which point a scratch or wound is made in the culture. This area is imaged over a 24-72 period to monitor the rate of wound closure. Cells with increased migratory capacity will close the wound faster and are indicative of a more mesenchymal state