Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta
Background: In our previous investigations of the role of the extracellular matrix (ECM) in promoting neurite growth we have observed that a permissive laminin (LN) substrate stimulates differential g
Skeletal muscle is composed of multinucleated myofibers, in which nuclei share the cytoplasm, and other cell types. Here, we performed single-nucleus RNA sequencing (snRNA-Seq) to determine the gene e