The study of alternative splicing events in human induced pluripotent stem cells from a Down's syndrome patient

We employed the GeneChip Human Transcriptome Array 2.0 (HTA 2.0) for the global gene analysis with hiPSCs from DS and healthy individuals. Examining differentially expressed genes (DEGs) in these groups and focusing on specific transcripts with Alternative Splicing. Total RNA of hiPSCs from DS and healthy individuals was extracted and amplified 100 ng of RNA per sample were then hybridized on GeneChip Human Transcriptome Array 2.0 (HTA 2.0) (Affymetrix, Thermofischer scientific). Differentially expressed genes (DEGs) and alternative splicing events in these groups were examined.