CIL (2012) CIL:38963, Drosophila, invertebrate nurse cell, oocyte, follicle cell. CIL. Dataset

In situ hybridizations showing the localization of bicoid (top), oskar (middle) and gurken (bottom) mRNAs in the stage 10 Drosophila egg chambers. These localizations define the anterior- posterior and dorsal-ventral axes of the embryo. The egg chambers consists of 15 nurse cells and a large oocyte (right), which is surrounded by a layer of somatic follical cells. Bicoid mRNA localises to anterior of the oocyte, directing where head and thorax of embryo develop. Posterior localisation of oskar mRNA directs formation of the pole plasm that contains the abdominal and germline determinants. Gurken is translated on the dorsal side, producing a signalling molecule that causes adjacent follical cells to define the embryo's dorsal-ventral axis.

现场杂交实验展示了在10期果蝇卵室中bicoid（顶部）、oskar（中部）和gurken（底部）mRNA的定位。这些定位确定了胚胎的前后轴和背腹轴。卵室由15个滋养细胞和一个大型卵母细胞（右侧）组成，卵母细胞周围环绕着一层体细胞毛囊细胞。bicoid mRNA在卵母细胞的前端定位，指导胚胎头部和胸部的发育。oskar mRNA的后端定位则引导形成包含腹部和生殖细胞决定因子的极质体。gurken在背侧翻译，产生一种信号分子，使相邻的毛囊细胞界定胚胎的背腹轴。