Performance characteristics of the LAMP assays compared with real-time PCR.

*Running time: time needed to complete the test with reliable detection of all samples above the limit of detection.
aAnalytical sensitivity was tested on a pure culture suspension of a single strain representing each phylotype (see Table 2). Analytical sensitivity differs between phylotypes. More detailed results are presented in Table 2 (egl LAMP assay) and Table S3 in File S1 (modified fliC LAMP assay) and Table S6 in File S1 (real time PCR).
bAnalytical sensitivity tested on DNA isolated from spiked tuber extracts (with DNA of strain NCPPB 4156 that belongs to phylotype IIB) was assessed on two serial dilutions.
**ND: Non-determined. Analytical sensitivity in spiked plant extract was not tested with the 16S rRNA LAMP assay because of cross reactivity observed; therefore validation was not completed for this assay.