Expression profile of RCC4 cells with YAP silencing

YAP is the one of the effectors of Hippo pathway. YAP promotes organ size growth as well as tumor metastasis. In this study, YAP is silencing in clear cell carcinoma cell line RCC4 to elucidate the downstream targets that promotes the resisntance toward erastin-induced ferroptosis. Human clear cell renal carcinoma cell line RCC4 were transfected with siRNA targeting YAP(siYAP) and compared with non-targeting siRNA control (siNT). All samples were prepared as biological triplicates. Total RNA was collected after 2 days of reverse transfection for cDNA synthesis. The influence of silencing YAP on gene expression was derived by zero transformation process, in which we compared the gene expression level with siYAP to the average of gene expression level in control (siNT) samples.